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Comprehensive Organic Chemistry Experiments for the Laboratory Classroom

This expansive and practical textbook contains organic chemistry experiments for teaching in the laboratory at the undergraduate level covering a range of functional group transformations and key organic reactions.The editorial team have collected contributions from around the world and standardized them for publication. Each experiment will explore a modern chemistry scenario, such as: sustainable chemistry; application in the pharmaceutical industry; catalysis and material sciences, to name a few. All the experiments will be complemented with a set of questions to challenge the students and a section for the instructors, concerning the results obtained and advice on getting the best outcome from the experiment. A section covering practical aspects with tips and advice for the instructors, together with the results obtained in the laboratory by students, has been compiled for each experiment. Targeted at professors and lecturers in chemistry, this useful text will provide up to dat...

Related Papers

Journal of Chemical Education

David Pursell , Mai Tsoi

This text provides students with a comprehensive organic chemistry laboratory experience that emphasizes Green Chemistry principles. The Organic Chemistry I laboratory portion of the text uses classical experiments as vehicles for technique development and spectroscopic analysis. The Organic Chemistry II laboratory text section encompasses a multistep synthesis project specifically designed to leverage the techniques and instrumentation acquired by students during their Organic Chemistry I Laboratory experience.

Chiara Ghiron

KRYSTEL IRIS DE CASTRO

Innovative Publisher

Dr Akhil Nagar

The stratagem for literature searching in organic and inorganic synthesis increasingly becomes easier with the computer database. However, the dilemma has now become how to select one procedure instead of other. For neophyte, this is a difficult task, deciding the reaction conditions to be carried out to have the best chance of success. This practical procedure collection intended to serve lab-mate by sharing author’s own experience through most commonly used experimental procedures from established groups, reviewed journals and/or patents. Under the title of each experimental procedure, brief commentaries are often offered which summarize the authors’ personal experience. For the final products, detailed spectral data are not given because they simply take up too much space. We had a good time putting together these experimental procedures. We have even been using the manuscript ourselves quite often. Hopefully, you will find it as useful. We welcome your critique.

Journal of Engineering Research

Fernando León Cedeño

Nick Zyabko

Edward McIntee

Paul Morgan

AIP Conference Proceedings

Asep Kadarohman

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Golden Book of Chemistry Experiments

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Resource Type: Virtual Labs

The Virtual Lab is an online simulation of a chemistry lab. It is designed to help students link chemical computations with authentic laboratory chemistry. The lab allows students to select from hundreds of standard reagents (aqueous) and manipulate them in a manner resembling a real lab. More information and offline downloads . Please scroll below to find our collection of pre-written problems, they have been organized by concept and ranked by difficulty.

Stoichiometry

The mole, molarity, and density, glucose dilution problem.

In this activity, students use the virtual lab to create a 0.025M glucose solution from a standard 1M glucose solution. First, they calculate the correct volumes of 1M glucose solution and water to mix together…

Acid Dilution Problem

In this activity, students use the virtual lab to create 500mL of 3M HCl solution from a concentrated stock solution of 11.6M HCl. They must first calculate the correct volumes of 11.6M HCl solution and water to…

Cola and Sucrose Concentration Problem

In this activity, students use the virtual lab to prepare a sucrose solution for a soda recipe. They next calculate the concentration of their solution in terms of molarity, percent mass and density. Finally, they…

Making Stock Solutions from Solids

In this activity, students use the virtual lab to create stock solutions starting from solid salts. Students must first calculate the correct amount of solid to make the solution. Next, they prepare the solution…

Identifying the Unknown Metal (Metals Density Problem)

In this activity, students use the virtual lab to identify an unknown metal by measuring its density and comparing their measurements to the densities of known metals.

Identifying an Unknown Liquid from its Density

In this activity students use the virtual lab to design an experiment to determine the identity of mislabeled bottles using the densities of the solutions inside.

Alcohol Density Problem

Determine the concentration of an alcohol solution from its density.

Reaction Stoichiometry and Limiting Reagents

Gravimetric determination of arsenic.

Set in the context of ground water contamination in Bangladesh, this stoichiometry and analytical chemistry activity examines the issues around identifying wells contaminated with arsenic. (Part of a larger online…

Determining Stoichiometric Coefficients

In this activity, students use the virtual lab to determine how 4 unknown substances react with each other including their stoichiometric coefficients.

Stoichiometry and Solution Preparation Problem

In this limiting reagents problem, students mix together solutions in different ratios in an attempt to produce a final solution that contains only 1 product.

Textbook Style Limiting Reagents Problems

Textbook-style practice limiting reagent exercises with that can be used as a way to "predict and check" your answers using the virtual lab.

Textbook Style Limiting Reagents Problem II

In this activity, students practice with experiments involving limiting reagents and the test their knowledge to determine the concentration of an unknown solution.

Predicting DNA Concentration

In this limiting reagents problem, students are given specific concentrations of DNA solutions and are asked to predict what products and reactants will remain after a specific volumes are mixed and reaction has…

Unknown Concentration of DNA Solution Problem

In this advanced limiting reagent problem, students use the virtual lab to determine the concentration of a solution of DNA by reacting it with known amounts of a fluorescent dye which binds to the DNA.

Thermochemistry

Energy and enthalpy, camping problem i.

In this part of the MRE scenario, students measure the enthalpy of a reaction.

Camping Problem II

In this part of the MRE scenario, students determine change in the enthalpy of a reaction as the concentration of reactants are varied.

ATP Reaction (Thermochemistry and Bonding)

Determine the enthalpy of the ATP reaction.

Determining the Heat of Reaction in Aqueous Solution

In this activity, students perform an experiment to determine the heat of a reaction.

Coffee Problem

Use the virtual lab to determine how much milk to add to hot coffee to reach the desired temperature

Measuring the heat capacity of an engine coolant.

As an analytical chemist at a company developing new engine coolants your task is to determine the heat capacity of a newly developed product and then to determine if its heat capacity is greater of less than that…

Measuring the heat capacity of an engine coolant II (Advanced version)

Measure and compare the heat capacity of an unknown liquid with an unknown density.

Camping Problem III

In this part of the MRE scenario, students create solutions that when mixed, increase to a certain temperature.

Heats of Reaction - Hess' Law

This activity provides a demonstration of Hess' Law using three reactions: the solubility NaOH in water, the solubility NaOH in HCl and the reaction of a solution of HCl and a solution of NaOH.

Equilibrium

Lechatlier's principle, cobalt chloride and lechatlier’s principle.

In this activity, students safely explore the equilibrium reaction of the cobalt chloride reaction.

Equilibrium Calculations

Dna binding problem.

In this activity, students explore equilibrium constants in biochemical systems by measuring the binding constant of a DNA-Dye reaction.

Acid-Base Chemistry

Strong acids and bases, strong acid and base problems.

Textbook-style strong acid and base problems that can be checked using the Virtual Lab.

Determination of the pH Scale by the Method of Successive Dilutions

This activity was created as an accompaniment to an in-class demonstration of the method of successive dilutions using HCl, NaOH, a pH meter, and universal indicator solution. After the demonstration, students…

Weak Acids and Bases

Weak acid and base problems.

Textbook-style weak acid and base problems that can be checked using the Virtual Lab.

Determining the pKa and Concentration Ratio of a Protein in Solution

Use the virtual lab to determine the pKa of a protein then create a buffer solution with a specific concentration ratio of the protein in its protonated/ unprotonated form.

Unknown Acid and Base Problem

In this exercise, students graph the titration curve of an unknown acid and base to determine their pKa’s and concentrations.

Buffer Solutions

Creating a buffer solution.

An exercise to design a buffer solution with specific properties.

DNA - Dye Binding: Equilibrium and Buffer Solutions

Students examine equilibrium and buffer solutions in a biological setting.

Acid/Base Titrations

Standardization of naoh with a khp solution: acid base titration.

Use the Virtual Laboratory to standardize an unknown NaOH solution (approximately 0.2M) to four significant figures via titration with 25.00 mL of a KHP standard solution.

Solubility Product

Determining the solubility product.

Determine the solubility product constatnt (Ksp) for various solids.

Temperature and the Solubility of Salts

Examine the solubilities of salts based on temperature.

Determining the solubility of copper chloride at different temperatures

GIven the solubility of CuCl at 2 different temperatures, predict its solubility at a third temperature. Then test your prediction by creating the solution in the virtual lab

Oxidation/Reduction and Electrochemistry

Standard reduction potentials, exploring oxidation-reduction reactions.

Design an experiment to order Cu, Mg, Zn and Pb from strongest to weakest reducing agent.

Analytical Chemistry/Lab Techniques

Gravimetric analysis, unknown silver chloride.

Determine the concentration of Silver ion in a Silver Nitrate solution using gravimetric analysis

The ChemCollective site and its contents are licensed under a Creative Commons Attribution 3.0 NonCommercial-NoDerivs License.

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Tips, ideas and practical experiments to help you make the most of microscale chemistry in your classroom

On this page

• Getting started

Why microscale?

Microscale chemistry practicals.

The fast guide to microscale practical work

By Maria Burke

Every chemistry teacher’s must-have guide to this no-fuss method for practical work

Apparatus and techniques for microscale chemistry

Find out what apparatus you need for common microscale experiments, learn about key techniques and discover how to prepare solutions of different elements.

Maximise learning with microscale

By Bob Worley

Find out why and how a small-scale approach to practical work reaps big rewards in your classroom

Making practical work more effective

By David Paterson

Microscale chemistry and well-ordered teaching sequences reduce cognitive overload

Why I love microscale

Advocating a practical approach to practical work in the classroom

More from Education in Chemistry

Escape the classroom: and try microscale chemistry

By Adrian Allan

Escape room puzzles based on simple microscale reactions for students

Exhibition Chemistry demonstrations

Engage and inspire your students with these exciting demonstrations, including videos, practical guidance and teaching notes

The movement of ions: bringing electrolysis to life

Demonstrate the movement of positive and negative ions with a simpler, safer version of this classic demo

Dynamite soap: The combustion of stoichiometric hydrogen–oxygen mixtures

Add this quick demo to the end of a lesson on squeaky pops to show the dramatic impact of mixing chemicals in the correct proportions

Practical videos

Video resources featuring microscale experiments, designed to support flipped learning and live practical work

Displacement reactions – practical videos | 14–16 students

Video and resources investigating the displacement reactions between the halogens chlorine, bromine and iodine and their respective halides in microscale

Identifying ions – practical videos | 14–16 students

Video resource showing how to identify ions in various solutions. Flame tests, sodium hydroxide test (microscale) and tests for negative ions: carbonate, sulfate and halide ions

Reactivity of metals | practical videos | 14–16 years

Video with supporting resources featuring three experiments investigating the relative reactivity of metals, including metal displacement reactions in microscale

Electrochemical cells | practical videos | 16–18 students

Video and supporting resources to support electrochemistry practical work, including two microscale experiments, animation and cell diagrams

More microscale experiments and demonstrations

Precipitation reactions of lead nitrate

Compare the colours of various lead compounds to identify which would be good pigments in this microscale practical. Includes kit list and safety instructions.

Some reactions of sulfur dioxide

Observe the reactions of sulfur dioxide with potassium manganate (IV), iodide/iodate mixture and indicator solution. Includes kit list and safety instructions. 

The determination of copper in brass

Try this microscale class practical to investigate how much copper there is in brass using nitric acid. Includes kit list and safety instructions.

Microscale reactions of hydrogen sulfide

Observe the reactions of hydrogen sulfide with lead nitrate, silver nitrate and potassium manganate(VII) in this microscale practical. Includes kit list and safety instructions.

Microscale reactions of ammonia

Try this practical to explore the reactions of ammonia with indicator solution, copper(II) sulfate solution and Nessler’s reagent. Includes kit list and safety instructions.

Measuring density

By measuring the relative mass of seawater and tap water, students will be able to discover the density of these liquids. Includes kit list and safety instructions. 

The chemistry of thiosulfate ions

Sodium thiosulfate has several interesting reactions with a variety of chemicals. This experiment will let students explore and record these reactions. Includes kit list and safety instructions.

Some reactions of nitrogen dioxide

Using a range of chemicals and solutions, students can create an experiment that will explore some of the reactions of nitrogen dioxide. Includes kit list and safety instructions.

Testing acids and bases on a microscale

Test various substances with indicator solution and look for colour changes in this microscale class practical. Includes kit list and safety instructions.

Mass changes in chemical reactions

Perform two chemical reactions to see whether any mass changes occur in this microscale class practical. Includes kit list and safety instructions.

The oxidation of cyclohexanol by nitric acid

Perform a ring opening oxidation using nitric acid to produce the dicarboxylic acid, 1,6-hexanedioic acid (adipic acid) – and then use the solid crystals that form to determine a melting point. Includes kit list and safety instructions.

Exploring the chemistry of chromium, molybdenum and tungsten

Discover how transition elements differ in aspects of colour, precipitate formation, changes in oxidation state and equilibria. Includes kit list and safety instructions.

Brady’s test for aldehydes and ketones

Identify aldehydes and ketones using Brady’s reagent (2,4-dinitrophenylhydrazine) in this microscale experiment. Includes kit list and safety instructions.

The chemical properties of phenol

Observe and interpret some of the chemical reactions of hydroxybenzene (phenol), by adding five different substances to a Petri dish, and noting down findings. Includes kit list and safety instructions.

Preparing ethyne on a microscale

Generate ethyne gas with calcium carbide and test its properties in this microscale class practical. Includes kit list and safety instructions.

Observing chemical changes

Try this microscale practical to explore the chemical changes in displacement, redox and precipitation reactions. Includes kit list and safety instructions.

Diffusion of gases on a microscale

Try this class practical to explore the diffusion of gases and how relative molecular mass affects rate of diffusion. Includes kit list and safety instructions.

Redox chemistry with dichromate ions

Observe the colour changes that occur with the reduction of dichromate ions by hydrogen peroxide. Includes kit list and safety instructions.

Oxidation states of iron

Compare the two main oxidation states of iron and consider explanations for differences in this microscale practical. Includes kit list and safety instructions.

Microscale reactions of metals with acids

Try this class practical to explore reactivity series with various metals as they react with acids on a microscale. Includes kit list and safety instructions.

Unsaturation test with potassium manganate(VII)

Use a solution of potassium manganate to test for unsaturation in organic compounds in this microscale practical. Includes kit list and safety instructions.

Properties of group 2 elements

Microscale experiment where various anion solutions are added to drops of group 2 element cations. Includes kit list and safety instructions.

Testing for unsaturation with bromine on a microscale

Try this class experiment to prepare elemental bromine and use it to test for unsaturation in organic compounds. Includes kit list and safety instructions.

Oxygen and methylene blue

Reacting hydrogen peroxide, and potassium manganate together will produce detectable oxygen so by using methylene blue solution, and a gas generating apparatus students can test for the presence of oxygen in this practical. Includes kit list and safety instruction.

Synthesis of aspirin on a microscale

Use this class practical to produce aspirin in a microscale esterification reaction using phosphoric acid as a catalyst. Includes kit list and safety instructions.

Energy changes in neutralisation

Study energy changes in two chemical reactions using thermometer strips to measure temperature in this experiment. Includes kit list and safety instructions.

Formation of TCP (2,4,6-trichlorohydroxybenzene)

Delve into preparing TCP by reacting hydroxybenzene (phenol) with chlorine gas, and create this distinctive smelling compound.

Investigating redox reactions on a microscale

Carry out two redox reactions and observe and interpret the results in this microscale class practical. Includes kit list and safety instructions.

The microscale synthesis of indigo dye

Carry out a microscale organic synthesis, the result of which will leave students with indigo dye. Includes kit list and safety instructions.

Finding out how much salt there is in seawater

Use the microscale titration apparatus to titrate silver nitrate solution against sea water

The treatment of oil spills

Tackle the real-life environment problem of oil spills in your classroom, by creating and then treating a micro version of an oil event. Includes kit list and safety instructions.

Some reactions of carbon dioxide

Create carbon dioxide from marble chips and acid, then test for its reaction with barium hydroxide by observing the carbonate precipitate. Includes kit list and safety instructions.

The microscale synthesis of azo dyes

Synthesise an azo dye, and use it to change the colour of cotton, with this class experiment. Includes kit list and safety instructions.

Sulfate and carbonate solubility of Groups 1 and 2

Try this microscale practical to explore the properties of elements in Groups 1 and 2 as they form various precipitates. Includes kit list and safety instructions.

Measuring an equilibrium constant on a microscale

Use your microscale titration apparatus to determine the equilibrium constant for the reaction between silver(I) and iron(II) ions

Exploring the properties of the carvones

Test the smell of each enantiomer of carvone and detect the differences

Measuring the amount of vitamin C in fruit drinks

Explore ascorbic acid in fruit drinks through titration in this experiment, with specimen results and calculations, stock solutions, and detailed notes included. 

Displacement reactions of metals on a microscale

Examine the reactions between various metals and metal salt solutions in this microscale class practical. Includes kit list and safety instructions.

Electrolysis using a microscale Hoffman apparatus

Investigate the electrolysis of sodium sulfate solution using a microscale Hoffman apparatus in this class practical. Includes kit list and safety instructions.

The chemistry of silver

Discover the properties of silver compounds with redox reactions, complex formation and colour/state changes. Includes kit list and safety instructions.

Transition elements and complex compounds microscale experiment | 16–18 years

Try this microscale practical investigating the transition elements, complex formation and change in oxidation state. Includes kit list and safety instructions

Analysis of aspirin tablets on a microscale

Try this microscale class practical to analyse aspirin tablets and find out how much salicylic acid is present. Includes kit list and safety instructions.

The temperature changes induced by evaporation

Explore the rate of evaporation for a trio of liquids, using just a temperature strip, and our worksheet. Includes kit list and safety instructions.

Properties of stereoisomers

By soaking cotton wool in two limonene enantiomers, and adding a stereoisomer, students can explore the differences between each chemical and discuss how they each might react in different conditions. Includes kit list and safety instructions.

Using a microscale conductivity meter

Explore electrical conductivity with this practical that allows students to test different materials for how well a current will pass through them. Includes kit list and safety instructions.

Microscale extraction of copper

In association with Nuffield Foundation

Try this practical to reduce copper(II) oxide to copper using hydrogen, revealing their positions in the reactivity series. Includes kit list and safety instructions.

Practical potions microscale | 11–14 years

Observe chemical changes in this microscale experiment with a spooky twist.

Microscale technicians in trouble! investigation

Some solutions have been mixed up – help the technicians work out which is which

About this collection

A number of the practicals and demonstrations featured on this page are based on experiments previously published in Microscale chemistry: experiments in miniature . Royal Society of Chemistry members receive a 35% discount at the RSC bookshop.

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Practical analytical chemistry lab, manual lab

• December 2018
• In book: Practical analytical chemistry lab, manual lab (pp.1-28)

• Mustansiriyah University

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