Electrophoretic Light Scattering
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- First Online: 27 April 2018
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- Jason CW Corbett 4 &
- Fraser McNeil-Watson 4
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Dynamic light scattering ; Electrical double layer (EDL) ; Laser Doppler electrophoresis (LDE) ; Light scattering ; Phase analysis light scattering (PALS)
Introduction
Electrophoretic light scattering, commonly abbreviated to ELS, is an analytical method that measures the electrophoretic mobility of particles or macromolecules in suspension . As in the related DLS (dynamic light scattering) method, the suspension is illuminated by a narrow light beam, generally a laser beam of a visible wavelength (∼400–800 nm). Under the application of an electric field, electrically charged entities in suspension will undergo directed motion toward their respective counter electrodes, depending on the sign of their overall charge, superimposed upon their randomly directed Brownian motion as measured using DLS. The directed component imparts a Doppler shift on the light scattered by the dispersed entities, from the illuminating light beam, which is collected by the optical system and from which...
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Akagi T et al (2006) Multifunctional conjugation of proteins on/into bio-nanoparticles prepared by amphiphilic poly(γ-glutamic acid). J Biomater Sci Polymer Edn 17(8):875–892
Article CAS Google Scholar
Corbett JCW (2017) Experimental progress in high conductivity electrophoresis using the diffusion barrier technique. http://www.malvern.com/en/support/resource-center/posters/PT150602HighConductElectrophoresis.aspx
Corbett JCW, Jack RO (2010) Measuring protein mobility using modern microelectrophoresis. Colloids Surf A 376(1–3):31–41
Google Scholar
Corbett JCW, Connah MT, Mattison K (2011) Advances in the measurement of protein mobility using laser Doppler electrophoresis – the diffusion barrier technique. Electrophoresis 32:1787–1794
Article CAS PubMed Google Scholar
Cummins HZ, Pike ER (eds) (1973) Photon correlation and light beating spectroscopy. Plenum, New York, p 314. isbn:0-306-35703-8
Cummins HZ, Pike ER (eds) (1976) Photon correlation spectroscopy and velocimetry. Plenum, New York, pp 260–373. isbn:0-306-35723-2
Garg A, Cartier CA, Bishop KJM, Velgool D (2016) Particle Zeta Potentials Remain Finite in Saturated Salt Solutions. Langmuir 32:11837–11844
Hiemenz PC, Rajagopalan R (1997) Principles of colloid and surface chemistry. CRC Press, New York. isbn:0-8247-9397-8
Hunter RJ (1981) Zeta potential in colloid science. Academic, London. isbn:0-12-361960-2
Chapter Google Scholar
Jachimska B, Wasilewska M, Adamczyk Z (2008) Characterization of globular protein solutions by dynamic light scattering, electrophoretic mobility, and viscosity measurements. Langmuir 24(13):6866–6872
Okuda T et al (2006) Biodistribution characteristics of amino acid dendrimers and their PEGylated derivatives after intravenous administration. J Control Release 114:69–77
Schatzel K, Wiese W, Sobotta A, Drewel M (1991) Electroosmosis in an oscillating field: avoiding distortions in measured electrophoretic mobilities. J Colloid Interface Sci 143:287–293
Article Google Scholar
Ware BR, Flygare WH (1971) The simultaneous measurement of the electrophoretic mobility and diffusion coefficient in bovine serum albumin solutions by light scattering. Chem Phys Lett 12(1):81
Ware BR, Flygare WH (1972) Light scattering in mixtures of BSA, BSA dimers, and fibrinogen under the influence of electricfields. J Colloid Interface Sci 39:3
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Jason CW Corbett & Fraser McNeil-Watson
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Correspondence to Fraser McNeil-Watson .
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University Leicester MRC Centre, Leicester, United Kingdom
Gordon Roberts
Dept Biochemistry, University of Oxford, Oxford, United Kingdom
Anthony Watts
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Stephen E. Harding
Mary Philips-Jones
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© 2018 European Biophysical Societies' Association (EBSA)
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Corbett, J.C., McNeil-Watson, F. (2018). Electrophoretic Light Scattering. In: Roberts, G., Watts, A. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35943-9_288-1
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DOI : https://doi.org/10.1007/978-3-642-35943-9_288-1
Received : 03 September 2017
Accepted : 18 March 2018
Published : 27 April 2018
Publisher Name : Springer, Berlin, Heidelberg
Print ISBN : 978-3-642-35943-9
Online ISBN : 978-3-642-35943-9
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