Electrophoretic Light Scattering

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electrophoretic light scattering experiments

  • Jason CW Corbett 4 &
  • Fraser McNeil-Watson 4  

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Dynamic light scattering ; Electrical double layer (EDL) ; Laser Doppler electrophoresis (LDE) ; Light scattering ; Phase analysis light scattering (PALS)

Introduction

Electrophoretic light scattering, commonly abbreviated to ELS, is an analytical method that measures the electrophoretic mobility of particles or macromolecules in suspension . As in the related DLS (dynamic light scattering) method, the suspension is illuminated by a narrow light beam, generally a laser beam of a visible wavelength (∼400–800 nm). Under the application of an electric field, electrically charged entities in suspension will undergo directed motion toward their respective counter electrodes, depending on the sign of their overall charge, superimposed upon their randomly directed Brownian motion as measured using DLS. The directed component imparts a Doppler shift on the light scattered by the dispersed entities, from the illuminating light beam, which is collected by the optical system and from which...

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Akagi T et al (2006) Multifunctional conjugation of proteins on/into bio-nanoparticles prepared by amphiphilic poly(γ-glutamic acid). J Biomater Sci Polymer Edn 17(8):875–892

Article   CAS   Google Scholar  

Corbett JCW (2017) Experimental progress in high conductivity electrophoresis using the diffusion barrier technique. http://www.malvern.com/en/support/resource-center/posters/PT150602HighConductElectrophoresis.aspx

Corbett JCW, Jack RO (2010) Measuring protein mobility using modern microelectrophoresis. Colloids Surf A 376(1–3):31–41

Google Scholar  

Corbett JCW, Connah MT, Mattison K (2011) Advances in the measurement of protein mobility using laser Doppler electrophoresis – the diffusion barrier technique. Electrophoresis 32:1787–1794

Article   CAS   PubMed   Google Scholar  

Cummins HZ, Pike ER (eds) (1973) Photon correlation and light beating spectroscopy. Plenum, New York, p 314. isbn:0-306-35703-8

Cummins HZ, Pike ER (eds) (1976) Photon correlation spectroscopy and velocimetry. Plenum, New York, pp 260–373. isbn:0-306-35723-2

Garg A, Cartier CA, Bishop KJM, Velgool D (2016) Particle Zeta Potentials Remain Finite in Saturated Salt Solutions. Langmuir 32:11837–11844

Hiemenz PC, Rajagopalan R (1997) Principles of colloid and surface chemistry. CRC Press, New York. isbn:0-8247-9397-8

Hunter RJ (1981) Zeta potential in colloid science. Academic, London. isbn:0-12-361960-2

Chapter   Google Scholar  

Jachimska B, Wasilewska M, Adamczyk Z (2008) Characterization of globular protein solutions by dynamic light scattering, electrophoretic mobility, and viscosity measurements. Langmuir 24(13):6866–6872

Okuda T et al (2006) Biodistribution characteristics of amino acid dendrimers and their PEGylated derivatives after intravenous administration. J Control Release 114:69–77

Schatzel K, Wiese W, Sobotta A, Drewel M (1991) Electroosmosis in an oscillating field: avoiding distortions in measured electrophoretic mobilities. J Colloid Interface Sci 143:287–293

Article   Google Scholar  

Ware BR, Flygare WH (1971) The simultaneous measurement of the electrophoretic mobility and diffusion coefficient in bovine serum albumin solutions by light scattering. Chem Phys Lett 12(1):81

Ware BR, Flygare WH (1972) Light scattering in mixtures of BSA, BSA dimers, and fibrinogen under the influence of electricfields. J Colloid Interface Sci 39:3

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Malvern Instruments Limited, Malvern, UK

Jason CW Corbett & Fraser McNeil-Watson

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Correspondence to Fraser McNeil-Watson .

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University Leicester MRC Centre, Leicester, United Kingdom

Gordon Roberts

Dept Biochemistry, University of Oxford, Oxford, United Kingdom

Anthony Watts

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Stephen E. Harding

Mary Philips-Jones

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© 2018 European Biophysical Societies' Association (EBSA)

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Corbett, J.C., McNeil-Watson, F. (2018). Electrophoretic Light Scattering. In: Roberts, G., Watts, A. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35943-9_288-1

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DOI : https://doi.org/10.1007/978-3-642-35943-9_288-1

Received : 03 September 2017

Accepted : 18 March 2018

Published : 27 April 2018

Publisher Name : Springer, Berlin, Heidelberg

Print ISBN : 978-3-642-35943-9

Online ISBN : 978-3-642-35943-9

eBook Packages : Springer Reference Biomedicine and Life Sciences Reference Module Biomedical and Life Sciences

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IMAGES

  1. Schematic representation of an electrophoretic light scattering (ELS

    electrophoretic light scattering experiments

  2. Electrophoretic light scattering

    electrophoretic light scattering experiments

  3. Understanding Electrophoretic Light Scattering Theory

    electrophoretic light scattering experiments

  4. Schematic of an apparatus for measurement of electrophoretic light

    electrophoretic light scattering experiments

  5. What is electrophoretic light scattering(ELS)?

    electrophoretic light scattering experiments

  6. Understanding Electrophoretic Light Scattering Theory

    electrophoretic light scattering experiments

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